Thermodynamic Parameters To Predict Stability of RNA/DNA Hybrid Duplexes
β Scribed by Sugimoto, Naoki; Nakano, Shu-ichi; Katoh, Misa; Matsumura, Akiko; Nakamuta, Hiroyuki; Ohmichi, Tatsuo; Yoneyama, Mari; Sasaki, Muneo
- Book ID
- 118735770
- Publisher
- American Chemical Society
- Year
- 1995
- Tongue
- English
- Weight
- 687 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-2960
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The rates of duplex formation for two octamers of DNA (5β² d-CACGGCTC/5β² d-GAGCCGTG and 5β² d-CACAGCAC/5β² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, ΞHΒ°and ΞSΒ°, have been de
Using nearest-neighbor models consisting of independent short sequence combinations of nearest neighbors (ISS models), values of thermodynamic parameters for sets of independent sequences are derived from published oligomer data for DNArRNA hybrids [N.