Kinetics and Thermodynamics of DNA, RNA,
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Rauzan, Brittany; McMichael, Elizabeth; Cave, Rachel; Sevcik, Lesley R.; Ostrosk
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Article
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2013
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American Chemical Society
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English
β 541 KB
The rates of duplex formation for two octamers of DNA (5β² d-CACGGCTC/5β² d-GAGCCGTG and 5β² d-CACAGCAC/5β² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, ΞHΒ°and ΞSΒ°, have been de