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Kinetics and Thermodynamics of DNA, RNA, and Hybrid Duplex Formation

✍ Scribed by Rauzan, Brittany; McMichael, Elizabeth; Cave, Rachel; Sevcik, Lesley R.; Ostrosky, Kara; Whitman, Elisabeth; Stegemann, Rachel; Sinclair, Audra L.; Serra, Martin J.; Deckert, Alice A.


Book ID
121331590
Publisher
American Chemical Society
Year
2013
Tongue
English
Weight
541 KB
Volume
52
Category
Article
ISSN
0006-2960

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Kinetics and Thermodynamics of DNA, RNA,
✍ Rauzan, Brittany; McMichael, Elizabeth; Cave, Rachel; Sevcik, Lesley R.; Ostrosk πŸ“‚ Article πŸ“… 2013 πŸ› American Chemical Society 🌐 English βš– 541 KB

The rates of duplex formation for two octamers of DNA (5β€² d-CACGGCTC/5β€² d-GAGCCGTG and 5β€² d-CACAGCAC/5β€² d-GTGCTGTG), the homologous RNA, and both sets of hybrids in 1 M NaCl buffer have been measured using stopped-flow spectroscopy. In addition, the thermodynamic parameters, Ξ”HΒ°and Ξ”SΒ°, have been de