Relative Thermodynamic Stability of DNA, RNA, and DNA:RNA Hybrid Duplexes: Relationship with Base Composition and Structure
โ Scribed by Lesnik, Elena A.; Freier, Susan M.
- Book ID
- 118735769
- Publisher
- American Chemical Society
- Year
- 1995
- Tongue
- English
- Weight
- 977 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0006-2960
No coin nor oath required. For personal study only.
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Using nearest-neighbor models consisting of independent short sequence combinations of nearest neighbors (ISS models), values of thermodynamic parameters for sets of independent sequences are derived from published oligomer data for DNArRNA hybrids [N.
## Background The high binding specificity of short 10 to 30 mer oligonucleotide probes enables single base mismatch (MM) discrimination and thus provides the basis for genotyping and resequencing microarray applications. Recent experiments indicate that the underlying principles governing DNA micr