Thermally Responsive Reversed Micelles for Immobilization of Enzymes
โ Scribed by H. Chen; L.-H. Liu; L.-S. Wang; C.-B. Ching; H.-W. Yu; Y.-Y. Yang
- Publisher
- John Wiley and Sons
- Year
- 2007
- Tongue
- English
- Weight
- 216 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1616-301X
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Trypsin and a-chymotrypsin were immobilized by gel entrapment in polyacrylamide cross-linked with N , Nmethylenebisacrylamide. The immobilized enzymes are catalytically efficient in suspensions of reverse micelles formed i n isooctane by bis(2-ethylhexyl) sodium sulfosuccinate) (AOT) and water. Both
## Synopsis A poly(viny1 alcohol) (PVA) fiber support incorporating various aminoacetal functional groups has been developed for immobilizing enzymes. The aminated PVA fiber seems to adsorb enzymes with electrostatic force of attraction; thus the immobilization procedure is simple. By the use of t