Fibrous support for immobilization of enzymes

✍ Scribed by Hisao Ichijo; Tetsuro Suehiro; Aizo Yamauchi; Shigeo Ogawa; Masanori Sakurai; Nobuo Fujii

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 1024 KB
• Volume: 27
• Category: Article
• ISSN: 0021-8995
• DOI: 10.1002/app.1982.070270523

✦ Synopsis

Synopsis

A poly(viny1 alcohol) (PVA) fiber support incorporating various aminoacetal functional groups has been developed for immobilizing enzymes. The aminated PVA fiber seems to adsorb enzymes with electrostatic force of attraction; thus the immobilization procedure is simple. By the use of this fiber having immobilized enzymes, the reaction between enzymes and substrates is nearly independent of the size of substrates. This newly developed type of fiber, which is formed by a mass superfine fibers (SFF), each measuring 1 pm or less in diameter, permits much more increased surface area than the conventional enzyme immobilization supports. Our studies of the properties of the fiber for immobilization of enzymes show the following results: (1) SFF has a greater ability for the immobilization of invertase than ordinary fibers; (2) dimethyl-aminated SFF has the best performance for the immobilization of invertase. From these results, it is concluded that the dimethyl-aminated SFF is an excellent support for the immobilization of invertase.