Characterization of sand as a support for immobilized enzymes

✍ Scribed by Jeffrey E. Brotherton; Alden Emery; Victor W. Rodwell

• Publisher: John Wiley and Sons
• Year: 1976
• Tongue: English
• Weight: 807 KB
• Volume: 18
• Category: Article
• ISSN: 0006-3592
• DOI: 10.1002/bit.260180407

✦ Synopsis

Abstract

The potential of sand as a support for immobilized enzymes was investigated by preparing alkylamine sand and devising methods to measure the total number of amine groups present and the fraction available for immobilization of enzymes. Alcohol dehydrogenase (alcohol:NAD oxidoreductase, EC 1.1.1.1) and lactate dehydrogenase (L‐lactate: NAD oxidoreductase, EC 1.1.1.27) were immobilized on alkylamine sand, and the stability of the immobilized protein and dehydrogenase activity was measured. Urease (urea amidohyrdrolase, EC 3.5.1.5) was also immobilized on sand to test the applicability of these methods to larger scale immobilizations. Results suggest that sand shows promise as a support for immobilized enzymes.