Thermal stability of the cellulose synthase complex of Acetobacter xylinum
โ Scribed by He Ping chen; R. Malcolm Brown
- Book ID
- 110272949
- Publisher
- Springer
- Year
- 1999
- Tongue
- English
- Weight
- 108 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0969-0239
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๐ SIMILAR VOLUMES
## Abstract Different methylated glucose derivatives and cellobiose were examined as the carbon sources for growth and cellulose formation by __Acetobacter xylinum__. HPLC studies were carried out to gain information about the kinetics of the utilization of the C sources used. The type and yields o
The gene for the catalytic subunit of cellulose synthase from Acetobacter xylinum has been cloned by using an oligonucleotide probe designed from the N-terminal amino acid sequence of the catalytic subunit (an 83 kDa polypeptide) of the cellulose synthase purified from trypsin-treated membranes ofA.
Three cellulose-negative (Cel-) mutants of Acetobacter xylinum strain ATCC 23768 were complemented by a cloned 2.8 kb DNA fragment from the wild type. Biochemical analysis of the mutants showed that they were deficient in the enzyme uridine 5'-diphosphoglucose (UDPG) pyrophosphorylase. The analysis