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Complementation of cellulose-negative mutants of Acetobacter xylinum by the cloned structural gene for phosphoglucomutase

✍ Scribed by Espen Fjærvik; Kirsten Frydenlund; Svein Valla; Yasser Huggirat; Moshe Benziman

Book ID
109316807
Publisher
John Wiley and Sons
Year
1991
Tongue
English
Weight
501 KB
Volume
77
Category
Article
ISSN
0378-1097

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Cloning of a gene involved in cellulose
Cloning of a gene involved in cellulose biosynthesis in Acetobacter xylinum: Complementation of cellulose-negative mutants by the UDPG pyrophosphorylase structural gene
✍ Valla, Svein ;Coucheron, Dag Hugo ;Fjærvik, Espen ;Kjosbakken, Johs ;Weinhouse, 📂 Article 📅 1989 🏛 Springer 🌐 English ⚖ 667 KB

Three cellulose-negative (Cel-) mutants of Acetobacter xylinum strain ATCC 23768 were complemented by a cloned 2.8 kb DNA fragment from the wild type. Biochemical analysis of the mutants showed that they were deficient in the enzyme uridine 5'-diphosphoglucose (UDPG) pyrophosphorylase. The analysis