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Kinetics of the utilization of different C sources and the cellulose formation by Acetobacter xylinum

✍ Scribed by U. Geyer; D. Klemm; H.-P. Schmauder

Publisher: John Wiley and Sons
Year: 1994
Tongue: English
Weight: 314 KB
Volume: 14
Category: Article
ISSN: 0138-4988
DOI: 10.1002/abio.370140308

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✦ Synopsis

Abstract

Different methylated glucose derivatives and cellobiose were examined as the carbon sources for growth and cellulose formation by Acetobacter xylinum. HPLC studies were carried out to gain information about the kinetics of the utilization of the C sources used. The type and yields of the synthesized cellulose were described. Besides glucose, cellobiose was a substrate for the synthesis of this polysaccharide by the bacteria. Other methylated derivatives of glucose were not accepted for a comparable synthesis of this polymer. An estimation of citrate in an unmodified culture liquid (SH medium) showed utilization in a late phase of cultivation. The influence of this organic acid on the pH value, cellulose synthesis and growth is described. By the application of citric acid as a sole carbon source “gel‐like” forms of cellulose were formed generally.

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