𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Thermal inactivation of immobilized penicillin acylase in the presence of substrate and products

✍ Scribed by Andres Illanes; Claudia Altamirano; Maria Elvira Zuñiga

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
787 KB
Volume
50
Category
Article
ISSN
0006-3592

No coin nor oath required. For personal study only.

✦ Synopsis

Inactivation of immobilized penicillin acylase has been studied in the presence of substrate (penicillin G) and products (phenylacetic acid and 6-aminopenicillanic acid), underthe hypothesis that substances which interact with the enzyme molecule during catalysis will have an effect on enzyme stability. The kinetics of immobilized penicillin acylase inactivation was a multistage process, decay constants being evaluated for the free-enzyme and enzyme complexes, from whose values modulation factors were determined for the effectors in each enzyme complex at each stage. 6-Aminopenicillanic acid and penicillin G stabilized the enzyme in the first stage of decay. Modulation factors in that stage were 0.96 for penicillin G and 0.98 for 6-aminopenicillanic acid. Phenylacetic acid increased the rate of inactivation in both stages, modulating factors being -2.31 and -2.23, respectively. Modulation factors influence enzyme performance in a reactor and are useful parameters for a proper evaluation. 0 1996

📜 SIMILAR VOLUMES

Electrically immobilized enzyme reactors
Electrically immobilized enzyme reactors: Bioconversion of a charged substrate. Hydrolysis of penicillin G by penicillin G acylase
✍ Alessandra Bossi; Stefania Guerrera; Pier Giorgio Righetti 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 136 KB 👁 2 views

The possibility of using the multicompartment immobilized enzyme reactor (MIER) in presence of a charged substrate is here explored. Penicillin G acylase is used to convert penicillin G (a free acid, with a pK of 2.6) into two charged products: phenyl acetic acid (PAA, with a pK of 4.2) and 6-aminop

Hydrolysis of Penicillin G by Penicillin
Hydrolysis of Penicillin G by Penicillin G Acylase Immobilized on Chitosan Microbeads in Different Reactor Systems
✍ M. G. Žuža; B. M. Obradović; Z. D. Knežević-Jugović 📂 Article 📅 2011 🏛 John Wiley and Sons 🌐 English ⚖ 711 KB

## Abstract The kinetic parameters for penicillin G hydrolysis in systems with penicillin G acylase from __Escherichia coli__ (free and immobilized on activated chitosan microbeads produced by electrostatic extrusion) were determined. The obtained kinetic results indicated that both systems (free a