Thermal elution chromatography and the resolution of nucleic acids on hydroxylapatite
โ Scribed by H.G. Martinson; E.B. Wagenaar
- Book ID
- 107711608
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 650 KB
- Volume
- 61
- Category
- Article
- ISSN
- 0003-2697
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## Abstract __Intercalating nucleic acids (INAยฎs) with insertions of (__R__)โ1โ__O__โ(1โpyrenylmethyl)glycerol were hybridized with locked nucleic acids (LNAs). INA/LNA duplexes were found to be less stable than the corresponding DNA/LNA duplexes when the INA monomer was inserted as a bulge close t
A fully automatized apparatus for studying the spectrophotometer-monitored thermal transition of nucleic acids is described. Measurements are made with improved accuracy over standard methods, under thermodynamically well-defined conditions (thermal and phase equilibrium). Examples of highly resol