A small-volume cuvette and holder makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 pg of DNA. The reduced sample size is especially important in the thermal characte~zation of DNA
An apparatus for studying the thermal transition of nucleic acids at high resolution
β Scribed by C. Reiss; F. Michel
- Publisher
- Elsevier Science
- Year
- 1974
- Tongue
- English
- Weight
- 618 KB
- Volume
- 62
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
A fully automatized apparatus for studying the spectrophotometer-monitored thermal transition of nucleic acids is described.
Measurements are made with improved accuracy over standard methods, under thermodynamically well-defined conditions (thermal and phase equilibrium).
Examples of highly resolved melting profiles of DNA are presented along with the general procedures for the computer treatment of experimental data.
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