𝔖 Bobbio Scriptorium
✦   LIBER   ✦

An apparatus for studying the thermal transition of nucleic acids at high resolution

✍ Scribed by C. Reiss; F. Michel


Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
618 KB
Volume
62
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis


A fully automatized apparatus for studying the spectrophotometer-monitored thermal transition of nucleic acids is described.

Measurements are made with improved accuracy over standard methods, under thermodynamically well-defined conditions (thermal and phase equilibrium).

Examples of highly resolved melting profiles of DNA are presented along with the general procedures for the computer treatment of experimental data.


πŸ“œ SIMILAR VOLUMES


Small-volume cuvette for high-resolution
✍ A.T. Ansevin; D.L. Vizard πŸ“‚ Article πŸ“… 1979 πŸ› Elsevier Science 🌐 English βš– 696 KB

A small-volume cuvette and holder makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 pg of DNA. The reduced sample size is especially important in the thermal characte~zation of DNA

Apparatus for studying the effects of ra
✍ Warren F. Savage πŸ“‚ Article πŸ“… 1962 πŸ› John Wiley and Sons 🌐 English βš– 1003 KB

## Abstract A new research tool is described which permits determination of short‐time elevated temperature properties of metals and alloys. Test specimens are heated by their own resistance to the flow of a large magnitude heating current, and a rapid‐acting electronic‐proportioning rate‐control a