✦ LIBER ✦

Small-volume cuvette for high-resolution thermal denaturation of nucleic acids

✍ Scribed by A.T. Ansevin; D.L. Vizard

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 696 KB
Volume: 97
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90337-3

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✦ Synopsis

A small-volume cuvette and holder makes it possible to obtain useful high-resolution thermal denaturation profiles on as little as submicrogram quantities of DNA and routine results with approximately 2 pg of DNA. The reduced sample size is especially important in the thermal characte~zation of DNA restriction fragments. If only the transition midpoint is needed, 0.05 pg of DNA can suffice. With the new microcuvette assembly, multiple samples may be determined simultaneously using a single-beam spectrophotometer equipped with a commercially available cuvette positioner.

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