Theoretical optimization of packed capillary column liquid chromatography using nonporous particles

✍ Scribed by Ying J. Yang; Milton L. Lee

• Publisher: John Wiley and Sons
• Year: 1999
• Tongue: English
• Weight: 157 KB
• Volume: 11
• Category: Article
• ISSN: 1040-7685
• DOI: 10.1002/(sici)1520-667x(1999)11:2<131::aid-mcs6>3.0.co;2-6

✦ Synopsis

The Horvath᎐Lin equation was modified to describe the peak dispersion in liquid chromatography for columns packed with nonporous spherical particles. The minimum separation times for compounds with different separation ratios were calculated by employing a series of fundamental equations for nonporous packings with different particle size. Particle diameter has an important Ž effect on separation speed. Considering a moderately high pressure limit e.g., 25

. MPa , short columns packed with small particles provide faster separations when separation ratios are large, while for difficult separations, longer columns must be used and the optimum particle size must be increased to minimize the separation time. Only at extremely high pressure will optimum particle size be extremely small. The calculations also show that the column length must be varied in order to minimize analysis time. The required length of column decreases as the particle size decreases for simple separations.