A molecular FRT (Flp recombinase recognition target)-based cassette system for multiple gene disruption in the yeast Saccharomyces cerevisiae was developed. FRT DNA sequences were designed with different core mutations and subsequently cloned in direct orientation upstream and downstream of a marker
The yeastSaccharomyces cerevisiae as a model organism for the cytoskeleton and cellbiology
β Scribed by Drubin, David
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 856 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0886-1544
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Yeast has great utility as a surrogate system to study aspects of mammalian biology. This utility extends to the drug discovery process, where yeast has been used to reveal the mechanism of action of compounds, to discover and characterize components of signaling pathways and to dissect protein func
The green fluorescent protein (GFP) of Aequorea victoria is now an established marker for gene expression and subcellular localization in budding yeast. Relatively high expression (greater than 2500 copies per cell) of GFP is required for direct microscopic visualization. This report provides a meth