The value of the local lymph node assay in quantitative structure-activity investigations
β Scribed by D. A. Basketter; D. W. Roberts; M. Crown
- Book ID
- 109374012
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 632 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0105-1873
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The local lymph node assay (LLNA), as recommended by the Interagency Coordinating Committee on the Validation of Alternative Methods (ICCVAM), only allows for the use of CBA mice. The objective of these studies was to begin to assess the response of chemical sensitizers in the LLNA across six strain
## Abstract The local lymph node assay (LLNA) is a skin sensitization test that provides animal welfare benefits. To reduce animal usage further, a modified version (rLLNA) was proposed. Conducting the rLLNA as a screening test with a single high dose group and vehicle control differentiated accura
A modified version of the local lymph node assay (LLNA) is presented, using bromodeoxyuridine to label proliferating lymphocytes. Cell counting is done on mid-sagittal sections of individual nodes under light microscopy. Two irritants (sodium lauryl sulfate and salicylic acid), four allergens of var