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The use of triphenyltetrazolium chloride for the quantitative analysis of sugars and sugar derivatives reported in glycoproteins

โœ Scribed by Jos Mes; L. Kamm

Book ID
104145527
Publisher
Elsevier Science
Year
1969
Tongue
English
Weight
622 KB
Volume
43
Category
Article
ISSN
1873-3778

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โœฆ Synopsis

Three different types of sugars, reported to be present in the carbohydrate moiety of glycoproteins, were estimated on paper chromatograms using 2,3,5-triphenyltetrazolium chloride. The use of steam for the visualization of the sugars on the chromatograms diminished the reaction time from the usual 15 min to z min with equal sensitivity and better reproducibility. linity and reaction time, established with series A, were selected for subsequent work in series B, Papers were dipped in a 0.5~/~ solution of TTC in 0.25 N NaOH in methanol and developed for z min in the steam chamber.

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