✦ LIBER ✦

The use of specimen tilt in transmission electron microscopy of the central nervous system

✍ Scribed by Milroy, Antonia M. ;Ralston, Diane D.

Publisher: Wiley (John Wiley & Sons)
Year: 1988
Tongue: English
Weight: 994 KB
Volume: 10
Category: Article
ISSN: 0741-0581
DOI: 10.1002/jemt.1060100109

No coin nor oath required. For personal study only.

✦ Synopsis

Thin sections of nervous tissue were viewed at different tilt angles using a transmission electron microscope equipped with a eucentric goniometer stage. In a comparison study of various degrees of tilt, one can observe additional morphological features within synaptic profiles, define subsynaptic structures such as Taxi-bodies, and clearly see the crystalline formation of cytochemical tracers. This study demonstrates the value of tilting thin-sections in the analysis of synapses and other biological material Synapse, Taxi-bodies, Horseradish peroxidase at the ultrastructural level.

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