The Use of Osazones as Matrices for the Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry of Carbohydrates

✍ Scribed by Peng Chen; Andrew G. Baker; Milos V. Novotny

• Publisher: Elsevier Science
• Year: 1997
• Tongue: English
• Weight: 136 KB
• Volume: 244
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1996.9874

✦ Synopsis

nic acid (11), a-cyano-4-hydroxycinnamic acid (12), 2-Fifteen sugar osazones were synthesized and evaluaminobenzoic acid (13), 3-hydroxypicolinic acid ( ), 3ated for their use as matrix-assisted laser desorption/ aminoquinoline (3-AQ) (15), 2,5-dihydroxybenzoic acid ionization matrices. Various carbohydrate samples were (DHB) ( ), and DHB/additive comatrices, including analyzed, indicating that D-or L-arabinosazone, pre-''super DHB'' (with 2-hydroxy-5-methoxybenzoic acid pared from D-or L-arabinose and phenylhydrazine, gave as an additive) (17) and DHB/HIQ (with 1-hydroxyisothe overall best performance. The arabinosazones faciliquinoline as an additive) (18).

tated superior analyses in providing a highly uniform

Osazones, the products of the reaction between a cardistribution of sample molecules within the matrix mibohydrate and an aromatic hydrazine, are among the crocrystals, and a possibility of using low laser energy best examples of crystallinity in organic chemistry. for irradiation, leading to high spectral resolution and Synthesized first in 1884 by Emil Fischer as a prelude low matrix background. The arabinosazones with a modto his remarkable studies on carbohydrate stereochemerate content of sodium ions promoted specific fragmenistry, osazones were used, for some time, in a microtation for certain carbohydrates tested. The 50 fmol scopic proof of carbohydrate structures.

amounts of maltohexaose and maltoheptaose (present in

During a search for suitable MALDI matrices for the 0.1 ml of sample/matrix solution) were detected at a sigcarbohydrate analysis, we became interested in osanal-to-background ratio of 3:1.