✦ LIBER ✦

Characterization of Two New Matrices for Matrix-assisted Laser Desorption/Ionization Mass Spectrometry

✍ Scribed by Jian Bai; Xiaoli Liang; Yan-Hui Liu; Yongdong Zhu; David M. Lubman

Publisher: John Wiley and Sons
Year: 1996
Tongue: English
Weight: 561 KB
Volume: 10
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(199605)10:7<839::aid-rcm579>3.0.co;2-c

No coin nor oath required. For personal study only.

✦ Synopsis

Three structurally related compounds, 4-hydroxy-3-methoxyphenylpyruvic acid (HMPPA), indole-3-pyruvic acid (IPA), and indole-3-glyoxylic acid have been evaluated as matrix-assisted laser desorption/ionization (MALDI) matrices. HMPPA and IPA were found to be effective matrices for MALDI-MS analysis of proteins and peptides and have been characterized in this work. HMPPA was found to be a particularly good matrix for the analysis of proteins and peptides because of its tolerance towards impurities and the resulting sensitivity in MALDI-MS experiments. Analysis of model proteins and peptides, including those in biological fluid, are demonstrated.

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has undergone continuous development

📜 SIMILAR VOLUMES

Polyamine co-matrices for matrix-assiste

Polyamine co-matrices for matrix-assisted laser desorption/Ionization mass spectrometry of oligonucleotides

✍ Victor E. Vandell; Patrick A. Limbach 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 110 KB 👁 2 views

The analysis of oligonucleotides using matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has led to the investigation of the use of matrix additives (i.e., co-matrices) to help improve the poor spectral quality commonly observed during the analysis of this class of compounds.

Comparison of rhodamine dyes as matrices

Comparison of rhodamine dyes as matrices for matrix-assisted laser desorption/ionization mass spectrometry

✍ K. Tang; S. L. Allman; R. B. Jones; C. H. Chen 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 383 KB

Comparison of Rhodamine Dyes as Matrices for ~a t n x -~i s t ~ Laser D ~o r p t i o n ~o n ~a t i o n ## Mass Spectrometry During the past few years, a tremendous effort has been applied to the mass spectrometric analysis of biopolymers. Hillenkamp and co-workers' developed the matrix-assisted

Structural characterization of polyoxyme

Structural characterization of polyoxymethylenes by matrix-assisted laser desorption/ionization mass spectrometry

✍ Hiroaki Sato; Hajime Ohtani; Shin Tsuge; Noriyuki Hayashi; Koichi Katoh; Eiji Ma 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB 👁 2 views

Several polyoxymethylene (POM) model compounds with relatively low molecular weights were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using dithranol matrix, 1,1,1,3,3,3-hexafluoro-2-propanol solvent for matrix/analyte solution, and sodium iodide as cati

Characterization of enzymatic pectin dig

Characterization of enzymatic pectin digests by matrix-assisted laser desorption/ionization mass spectrometry

✍ Körner, Roman; Limberg, Gerrit; Mikkelsen, Joørn Dalgaard; Roepstorff, Peter 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 265 KB 👁 2 views

The use of matrix-assisted laser desorption/ionization time-of-Ñight mass spectrometry for the characterization of partially methyl-esteriÐed enzymatic pectin digests is described. The sensitivities of several matrices, positive and negative ion modes and desalting techniques for these acidic oligos

Chemical reactivity in matrix-assisted l

Chemical reactivity in matrix-assisted laser desorption/ionization mass spectrometry

✍ C. Enjalbal; B. Sauvagnat; F. Lamaty; R. Lazaro; J. Martinez; P. Mouchet; F. Rou 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 2 views

During the control of a multistep organic synthesis on a soluble polymer (PEG) by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a chemical reactivity was encountered when the matrix was acidic, for the samples where the amino moiety of the anchored compounds was protected as

Matrix-assisted Laser Desorption/Ionizat

Matrix-assisted Laser Desorption/Ionization Mass Spectrometry in Milk Science

✍ Silvia Catinella; Pietro Traldi; Costante Pinelli; Evaristo Dallaturca; Renzo Ma 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 798 KB

The protein content of several cow milks was investigated by matrix-assisted laser desorptionhonization (MALDI) mass spectrometry. In particular, milk from four dinerent breeds of cow (Holstein Frisons, Brown Swiss, Jersey and Reggiana) at the same lactation stage and under the same feeding system w