𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Characterization of enzymatic pectin digests by matrix-assisted laser desorption/ionization mass spectrometry

✍ Scribed by Körner, Roman; Limberg, Gerrit; Mikkelsen, Joørn Dalgaard; Roepstorff, Peter

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
265 KB
Volume
33
Category
Article
ISSN
1076-5174

No coin nor oath required. For personal study only.

✦ Synopsis

The use of matrix-assisted laser desorption/ionization time-of-Ñight mass spectrometry for the characterization of partially methyl-esteriÐed enzymatic pectin digests is described. The sensitivities of several matrices, positive and negative ion modes and desalting techniques for these acidic oligosaccharides were compared. The most favorable results were obtained with a thin-layer preparation of a mixture of 2,4,6-trihydroxyacetophenone and nitrocellulose in the negative ion mode. Results are presented demonstrating the sensitive characterization of separated and unseparated high-ester pectin digests obtained after complete digestion using Aspergillus niger pectin lyase and the analysis of digests after chemical modiÐcation. In the case of unseparated digests, the analysis of methylation patterns is demonstrated. Oligomers with a degree of polymerization up to 40 were detected after enrichment of large oligomers by propan-2-ol precipitation.

📜 SIMILAR VOLUMES

Structural characterization of polyoxyme
Structural characterization of polyoxymethylenes by matrix-assisted laser desorption/ionization mass spectrometry
✍ Hiroaki Sato; Hajime Ohtani; Shin Tsuge; Noriyuki Hayashi; Koichi Katoh; Eiji Ma 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 161 KB 👁 2 views

Several polyoxymethylene (POM) model compounds with relatively low molecular weights were characterized by matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) using dithranol matrix, 1,1,1,3,3,3-hexafluoro-2-propanol solvent for matrix/analyte solution, and sodium iodide as cati

Mass Spectrometry of Low Molecular Mass
Mass Spectrometry of Low Molecular Mass Solids by Matrix-assisted Laser Desorption/Ionization
✍ Goheen, Steven C.; Wahl, Karen L.; Campbell, James A.; Hess, Wayne P. 📂 Article 📅 1997 🏛 John Wiley and Sons 🌐 English ⚖ 325 KB 👁 2 views

Matrix-assisted laser desorption/ionization combined with time-of-Ñight mass spectrometry (MALDI/TOF-MS) was used for the analysis of low molecular mass compounds. Three classes of molecules were studied : organic acids, salts of oxyanions and amine-based chelating compounds. Mass spectra from sampl

Quantitative Analysis of Oligonucleotide
Quantitative Analysis of Oligonucleotides by Matrix-assisted Laser Desorption/Ionization Mass Spectrometry
✍ Bernd A. Bruenner; Tai-Tung Yip; T. William Hutchens 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 434 KB 👁 2 views

Quantitative aspects of oligonucleotide analysis by matrix-assisted laser desorption/iotion (MALDI) mass spectrometry remain largely unexplored relative to the efforts that have been devoted to quantitative peptide and protein analysis. The successful quantitation of these other biopolymers coupled

Chemical reactivity in matrix-assisted l
Chemical reactivity in matrix-assisted laser desorption/ionization mass spectrometry
✍ C. Enjalbal; B. Sauvagnat; F. Lamaty; R. Lazaro; J. Martinez; P. Mouchet; F. Rou 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 139 KB 👁 2 views

During the control of a multistep organic synthesis on a soluble polymer (PEG) by matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, a chemical reactivity was encountered when the matrix was acidic, for the samples where the amino moiety of the anchored compounds was protected as

Differentiation of enantiomers using mat
Differentiation of enantiomers using matrix-assisted laser desorption/ionization mass spectrometry
✍ M. P. So; T. S. M. Wan; T.-W. Dominic Chan 📂 Article 📅 2000 🏛 John Wiley and Sons 🌐 English ⚖ 54 KB 👁 2 views

Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has successfully been used to differentiate pseudo-enantiomeric (isotopically labelled) amino acids by using cyclodextrin as complexing host. By using different pseudo-enantiomeric mixtures (i.e. R Dn S; and R S Dn ), it has be