The use of o-phthaldialdehyde in the detection of proteins and peptides
β Scribed by A.R. Torres; V.L. Alvarez; L.B. Sandberg
- Book ID
- 113125080
- Publisher
- Elsevier Science
- Year
- 1976
- Weight
- 276 KB
- Volume
- 434
- Category
- Article
- ISSN
- 0005-2795
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
Amino acids and peptides separated by ~hromatog~phy and eIe~trophoresis on thin layers of ceIIuiose or silica gel were located by spraying them with either ninhydrin, fluorescamine, orb-ph~aldiaIdehyde. o-Phthaldialdehyde and ninhydrin permitted the detection of amino acids in the SO-ZOO-pmo1 range
Using a variety of synthetic peptides, it was shown that the reaction of o-phthalaldehyde with peptides to yield fluorescent derivatives was dependent upon the presence of the free l -akin0 group of lysine. Procedures for the analysis of mixtures of amino acids have recently been increased in sensi
The potential application of the o-phthaldialdehyde (OPA) reagent for quantification of the type and extent of the reaction(s) catalysed by transglutaminase (TGase) during incubation with sodium caseinate (NaCN) was investigated. Initial studies were performed to ensure that NH3, a by-product of TGa