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Rapid and sensitive location of protein zones in gels using o-phthaldialdehyde

โœ Scribed by Michael D. Taylor; Anthony T. Andrews


Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
570 KB
Volume
2
Category
Article
ISSN
0173-0835

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The use of the o-phthalaldehyde reaction
โœ Stanley A. Robrish; Christopher Kemp; William H. Bowen ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 618 KB

Alkaline hydrolysis of protein followed by reaction with o-phthalaldehyde has permitted the determination of less than 10 ng of protein fluorometrically. When intact proteins were analyzed with o-phthalaldehyde the reaction was less sensitive. This reaction has been applied to analysis of the protei