High-performance liquid chromatographic determination of amino acids in protein hydrolysates and in plasma using automated pre-column derivatization with o-phthaldialdehyde/2-mercaptoethanol
β Scribed by G. Georgi; C. Pietsch; G. Sawatzki
- Publisher
- Elsevier Science
- Year
- 1993
- Weight
- 626 KB
- Volume
- 613
- Category
- Article
- ISSN
- 0378-4347
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π SIMILAR VOLUMES
An analytical method for a rapid reverse-phase liquid chromatography of amino acids is presented. The total analysis time was 19 min. The average relative deviation over the measured peak area was lower than 10% and the fluorescent response was linear with concentration for OPA derivatives (r > 0.99
## Abstract A highly sensitive and selective HPLC method with UV detection was developed for the determination of cefepime in goat plasma and milk. The proposed method was based on the complexation of cefepime with Hg(I) ions that imparts the high selectivity of the proposed method with enhancement