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The use of nanosecond fluorometry in detecting conformational transitions of an allosteric enzyme

✍ Scribed by Ming S. Tung; Robert F. Steiner

Publisher
Wiley (John Wiley & Sons)
Year
1975
Tongue
English
Weight
807 KB
Volume
14
Category
Article
ISSN
0006-3525

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✦ Synopsis

Abstract

Analysis of the time decay of fluorescence of 1‐anilino‐naphthalene‐8‐sulfonate bound to phosphorylase b reveals the presence of two components with different decay times. In glycylglycine buffer, pH 7.0, the addition of the activators AMP or IMP, or the conversion to phosphorylase a results in a preferential decrease in the amplitude of the component of longer decay time, arising probably from a preferential dissociation of 1‐anilino‐naphthalene‐8‐sulfonate from the corresponding site as a consequence of a change in molecular state.

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