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An enzyme-linked immunosorbent assay for the detection of antitetanus toxoid antibody using aluminum-absorbed coating antigen

✍ Scribed by G. M. Thiele; J. Rogers; M. Collins; D. Smith; T. L. McDonald; N. Yasuda

Publisher
John Wiley and Sons
Year
1990
Tongue
English
Weight
375 KB
Volume
4
Category
Article
ISSN
0887-8013

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✦ Synopsis

Abstract

An enzyme‐linked immunosorbent assay (ELISA) for the detection of antitetanus antibodies was developed using aluminum‐absorbed tetanus toxoid as the coating antigen. The assay was tested by measuring antitetanus antibody levels in serum obtained from subjects before and after immunization with the aluminum‐absorbed tetanus suspension. The specificity of the antibodies for tetanus antigen was tested both before and after antitetanus activity was removed with tetanus antigen–coated beads. Also, the activities of the sera were compared with those of a commercially available test. Our results indicated that the aluminum‐absorbed tetanus suspension can be coated onto standard polystyrene ELISA plates and used to measure antibody titers to tetanus toxoid.

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