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Development of an enzyme-linked immunosorbent assay for the detection of ciguatoxin in fish tissue using chicken immunoglobulin Y

✍ Scribed by Cara Empey Campora; Yoshitsugi Hokama; Kenichi Yabusaki; Minoru Isobe

Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
168 KB
Volume
22
Category
Article
ISSN
0887-8013

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✦ Synopsis

Abstract

A sandwich enzyme‐linked immunosorbent assay was developed to detect ciguatoxin (CTX) in fish tissue. The assay utilizes two antibodies, chicken immunoglobulin Y specific to the ABCD domain of CTX and a mouse monoclonal immunoglobulin G–hor‐seradish peroxidase conjugate specific to the JKLM domain of CTX. The sensitivity, working range, cross reactivity, accuracy, precision, and reproducibility were examined. J. Clin. Lab. Anal. 22:239–245, 2008. © 2008 Wiley‐Liss, Inc.

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