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The use of immobilized enzyme-membrane sandwich reactors in automated analysis

โœ Scribed by J. Campbell; A.S. Chawla; T.M.S. Chang

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
307 KB
Volume
83
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

Glucose oxidase from A. niger (EC 1.1.3.4) was physically entrapped between two dialysis membranes. The resultant enzyme-membrane sandwich reactors were employed in a standard Technicon dialyzer unit at 37ยฐC and were found to perform efficiently in the automated analysis of aqueous glucose standards in the range 2.5-50 mg%. Over a lo-day period with intermittent usage, slight increases in the activities of the reactors were observed. The potential of such reactors in automated analysis is discussed. EXPERIMENTAL Type V glucose oxidase (Sigma Chemical Co.) with 1400 units/ml was used without further purification.

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โœ S. Cliffe; C. Filippini; M. Schneider; M. Fawer ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 396 KB

Membrane-type reactors were prepared usmg a commercially available support In three test systems, mvolvmg glucose oxldase, glutammase and lactase, they compared favourably with packed-bed reactors m a flow-mJectlon set-up Although slmdar responses were observed for all reactor types, the membranes a