Glucose oxidase from A. niger (EC 1.1.3.4) was physically entrapped between two dialysis membranes. The resultant enzyme-membrane sandwich reactors were employed in a standard Technicon dialyzer unit at 37Β°C and were found to perform efficiently in the automated analysis of aqueous glucose standards
Membrane-type immobilized enzyme reactors for use in flow-injection analysis
β Scribed by S. Cliffe; C. Filippini; M. Schneider; M. Fawer
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 396 KB
- Volume
- 256
- Category
- Article
- ISSN
- 0003-2670
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β¦ Synopsis
Membrane-type reactors were prepared usmg a commercially available support In three test systems, mvolvmg glucose oxldase, glutammase and lactase, they compared favourably with packed-bed reactors m a flow-mJectlon set-up Although slmdar responses were observed for all reactor types, the membranes are easier to handle than mlcropartlcles When makmg contmuous m-hne measurements of bloprocesses at high samphng frequencies, the hfetlmes of packed beds of controlled pore glass were determmed by pressure budd-up wlthm the reactor When replaced wrth membrane reactors, no cloggmg was observed, the hfetlmes bemg determmed by loss of enzyme actlvlty Keywords Flow system, Enzyme reactor, Membrane reactor
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A spectrophotometric, continuous flow-injection, merging zones manifold including an enzyme reactor was used for the determination of magnesium ion in serum by exploiting the activating effect of MgfII) on the hydrolysis of o-nitrophenyl-B-o-galactopyranoside by j?-o-galactosidase immobiliied on con