## Abstract The use of fluorescent cyanine dyes to estimate membrane potential in cell suspensions has been considered. Several problems related to the application of the dyes have been reviewed. These problems include: (1) alteration of the membrane potential (E~m~) and factors involved in establi
The use of fluorescent dyes to measure membrane potentials: A critique
โ Scribed by R. M. Johnstone; P. C. Laris; A. A. Eddy
- Publisher
- John Wiley and Sons
- Year
- 1982
- Tongue
- English
- Weight
- 531 KB
- Volume
- 112
- Category
- Article
- ISSN
- 0021-9541
No coin nor oath required. For personal study only.
โฆ Synopsis
Abstract
Under controlled conditions, fluorescent cyanine dyes can be used to mesure membrane potentials of cell suspensions. Similar changes in membrane potential can be followed both with fluorescent dyes and electrophysiological probes in response to changes in the ion composition of the medium. Recent reports that attempt to abrogate the use of the cyanine dyes in measurements of the membrane potential are misleading.
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