✦ LIBER ✦

The use of flurescent dyes to measure membrane potentials: A response

✍ Scribed by Thomas C. Smith

Publisher: John Wiley and Sons
Year: 1982
Tongue: English
Weight: 552 KB
Volume: 112
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1041120222

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✦ Synopsis

Abstract

The use of fluorescent cyanine dyes to estimate membrane potential in cell suspensions has been considered. Several problems related to the application of the dyes have been reviewed. These problems include: (1) alteration of the membrane potential (E~m~) and factors involved in establishing E~m~ by the dyes themselves, (2) the effect of altered energy metabolism on the fluorescent response of the dyes and on E~m~, and (3) calibration of dye fluorescence. Recent reports that advocate the use of the fluorescent dyes are misleading.

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