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Use of an encapsulated fluorescent probe to measure intracellular PO2

✍ Scribed by Gary T. Podgorski; Ian S. Longmuir; James A. Knopp; Douglas M. Benson

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
470 KB
Volume
107
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

The objectives of this investigation were to produce a reliable, senstive probe to measure intracellular P~O2~ with a high degree of resolution and to apply this technique to biological systems. A fluorescent molecule, pyrene dissolved in paraffin oil, was encapsulated in polyacrylamide to form a probe of nanometer dimensions. The quantitative and microscopic oxygen values were determined by analyzing the quenching of the fluorescence of the probe by oxygen, as displayed on a television monitor by a silicon‐intensified‐target camera. The nanocapsules had a sensitivity of approximately 1 mm P~O2~, a spatial resolution of 0.5 ΞΌm, and a temporal resolution of milliseconds. Calibrated nanocapsules within nonrespiring Amoeba proteus responded to ambient partial pressures of oxygen. At two different ambient partial pressures, nanocapsules engulfed by respiring amoebas indicated an intracellular P~O2~ 28 mm Hg less than extracellular P~O2~. The capsules retained their sensitivity to oxygen for at least 8 months.

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