The Use of Biotinylated Poly(ADP-Ribose) for Studies on Poly(ADP-Ribose)-Protein Interaction
β Scribed by F.M. Narendja; G. Sauermann
- Book ID
- 102559219
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 411 KB
- Volume
- 220
- Category
- Article
- ISSN
- 0003-2697
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Methods are described for the radiolabeling and determination of NAD+, poly(ADP-ribose), and protein-bound monomers of ADP-ribose in cultured mammalian cells. The adenine nucleotide pools of confluent monolayer cell cultures are radiolabeled using high-specific-activity [3H]adenine. Following any de
## Abstract We investigated the physical association of the DNA topoisomerase IIΞ² binding protein 1 (TopBP1), involved in DNA replication and repair but also in regulation of apoptosis, with poly(ADPβribose) polymeraseβ1 (PARPβ1). This enzyme plays a crucial role in DNA repair and interacts with ma