Methods are described for the radiolabeling and determination of NAD+, poly(ADP-ribose), and protein-bound monomers of ADP-ribose in cultured mammalian cells. The adenine nucleotide pools of confluent monolayer cell cultures are radiolabeled using high-specific-activity [3H]adenine. Following any de
✦ LIBER ✦
Immunodot Blot Method for the Detection of Poly(ADP-ribose) Synthesizedin Vitroandin Vivo
✍ Scribed by El Bachir Affar; Patrick J. Duriez; Rashmi G. Shah; Frédéric R. Sallmann; Sylvie Bourassa; Jan-Heiner Küpper; Alexander Bürkle; Guy G. Poirier
- Publisher
- Elsevier Science
- Year
- 1998
- Tongue
- English
- Weight
- 160 KB
- Volume
- 259
- Category
- Article
- ISSN
- 0003-2697
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did not appear to migrate out of the gel, causing band aggregation toward the bottom. We also used the Spreadex gels in conventional gel tanks such as the BRL Horizon 11.14. without buffer recirculation or temperature control; results were much inferior to those obtained with the instrument used he