The Ti-plasmid of Agrobacterium tumefaciens: a natural genetic engineer
โ Scribed by Hooykaas, P.J.J.; Schilperoort, R.A.
- Book ID
- 123264322
- Publisher
- Elsevier Science
- Year
- 1985
- Tongue
- English
- Weight
- 308 KB
- Volume
- 10
- Category
- Article
- ISSN
- 0968-0004
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant
The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transf