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The Ti-plasmid of Agrobacterium tumefaciens: a natural genetic engineer

โœ Scribed by Hooykaas, P.J.J.; Schilperoort, R.A.


Book ID
123264322
Publisher
Elsevier Science
Year
1985
Tongue
English
Weight
308 KB
Volume
10
Category
Article
ISSN
0968-0004

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Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant

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The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transf