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Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region

✍ Scribed by Lundquist, Ronald C. ;Close, Timothy J. ;Kado, Clarence I.

Publisher: Springer
Year: 1984
Tongue: English
Weight: 798 KB
Volume: 193
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00327406

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✦ Synopsis

Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant TiC58 plasmid and a recombinant plasmid containing either the homologous wild-type DNA region or the homologous region containing a second Tn5 insertion. The analysis identified six complementation groups. Mutations in one of these complementation groups were not complemented in trans and represent a cis-dominant locus. The mutation in one complementation group showed variation in host range.

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