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In vivo transfer of the Ti-plasmid of Agrobacterium tumefaciens to Escherichia coli

โœ Scribed by Holsters, M. ;Silva, B. ;Van Vliet, F. ;Hernalsteens, J. P. ;Genetello, C. ;Van Montagu, M. ;Schell, J.

Publisher
Springer
Year
1978
Tongue
English
Weight
357 KB
Volume
163
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

The Ti-plasmids are naturally self-transmissible from their normal host Agrobacterium to E. coli. They are however unable to stably establish themselves as a replicon in E. coli. It is nevertheless possible to study the Ti-plasmids in E. coli with the help of Ti::RP4 cointegrate plasmids that transfer and maintain themselves very efficiently in E. coli. An E. coli harbouring such a Ti::RP4 plasmid is unable to catabolize octopine and unable to induce crown-gall tumours on plants.

๐Ÿ“œ SIMILAR VOLUMES

Genetic complementation of Agrobacterium
Genetic complementation of Agrobacterium tumefaciens Ti plasmid mutants in the virulence region
โœ Lundquist, Ronald C. ;Close, Timothy J. ;Kado, Clarence I. ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 798 KB

Mutants with Tn5 insertions in the vir region of the Agrobacterium tumefaciens TiC58 plasmid are unable to form crown-gall tumors. Complementation tests of these vir region mutants were carried out by constructing merodiploids in a recombination-deficient strain. Each merodiploid possessed a mutant