The TEACL method of DNA-DNA hybridization: Technical considerations
โ Scribed by Jeffrey R. Powell; Adalgisa Caccone
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 484 KB
- Volume
- 30
- Category
- Article
- ISSN
- 0022-2844
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## Abstract DNA from bacteriophage T7 was irradiated at long ultraviolet wavelengths in the presence of silver ions. Such treatment leads to selective production of thymine: thymine dimers in DNA. The DNA was melted and the renaturation rate was determined as a function of thymine dimer content and
Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybrid