✦ LIBER ✦

Hybridization method for quantitation of replicating polyoma DNA sequences

✍ Scribed by Unni Spaeren; Waldemar Waldeck; Rolf Eliasson

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 606 KB
Volume: 96
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90595-5

No coin nor oath required. For personal study only.

✦ Synopsis

Polyoma DNA was cleaved with restriction endonuclease HpaII, the fragments were separated by gel electrophoresis and transferred in good yield to separate nitrocellulose filters by a modification of the procedure of E. M. Southern (1975,J. Mol. Biol. 98,503-517.) The filters were then used in hybridization experiments to localize the isotope in different parts of the polyoma genome after in vitro incorporation of labeled deoxyribonucleoside triphosphates into the DNA.

📜 SIMILAR VOLUMES

Deletion analysis of minimal sequence re

Deletion analysis of minimal sequence requirements for autonomous replication of ors8, a monkey early-replicating DNA sequence

✍ Andrea Todd; Suzanne Landry; Christopher E. Pearson; Viviane Khoury; Dr. Maria Z 📂 Article 📅 1995 🏛 John Wiley and Sons 🌐 English ⚖ 890 KB

We have generated a panel of deletion mutants of ors8 (483 bp), a mammalian autonomously replicating DNA sequence, previously isolated by extrusion of nascent monkey (CV-1) DNA from replication bubbles active at the onset of S phase. The deletion mutants were tested for replication function by the D

Quantitative and qualitative analysis of

Quantitative and qualitative analysis of amplified DNA sequences by a competitive hybridization assay

✍ B. Térouanne; P. Balaguer; A.M. Boussioux; J.C. Nicolas 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 872 KB

The presence of allelic sequence variations in DNA fragments can be easily detected by measuring the extent of DNA strand exchange between test double-stranded PCR products (target) and labeled standard double-stranded PCR products (probe). Under selected hybridization conditions, sequences identica

Quantitative data analysis methods for b

Quantitative data analysis methods for bead-based DNA hybridization assays using generic flow cytometry platforms

✍ S. R. Corrie; G. A. Lawrie; B. J. Battersby; K. Ford; A. Rühmann; K. Koehler; D. 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 401 KB

## Abstract Bead‐based assays are in demand for rapid genomic and proteomic assays for both research and clinical purposes. Standard quantitative procedures addressing raw data quality and analysis are required to ensure the data are consistent and reproducible across laboratories independent of fl

Detection of virus-specific DNA sequence

Detection of virus-specific DNA sequences by quantitative in situ hybridization during infection with SV40

✍ Yves Langelier; Rosemonde Mandeville; René Simard 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 880 KB

## Abstract In situ hybridization has been used as a quantitative method to study the replication of SV40 during lytic infection. Various parameters have been defined such as fixation and denaturation of cytological preparations, concentration of complementary (^3^H)‐cRNA and volume of buffer used

Sequence-Independent Method forin VitroG

Sequence-Independent Method forin VitroGeneration of Nested Deletions for Sequencing Large DNA Fragments

✍ Arkady F. Fradkov; Konstantin A. Lukyanov; Mikhail V. Matz; Luda B. Diatchenko; 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 192 KB

Simple preparation method of PCR fragmen

Simple preparation method of PCR fragments for automated DNA sequencing

✍ Estrid Høgdall; Kit Boye; Jens Vuust 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 267 KB 👁 3 views

In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method w