Simple preparation method of PCR fragments for automated DNA sequencing
✍ Scribed by Estrid Høgdall; Kit Boye; Jens Vuust
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 267 KB
- Volume
- 73
- Category
- Article
- ISSN
- 0730-2312
No coin nor oath required. For personal study only.
✦ Synopsis
In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method works with greater success than the method compared. Our precipitation method may be used on a larger PCR fragment before cycle sequencing reaction as well. Furthermore, it has the advantage of being simple as the well-known dilution method; in contrast to the dilution method, the precipitation method removes excess primers as well as possible primer dimers.