## Abstract Immobilized and fully protected oligodeoxynucleotides containing a 4β(1,2,4βtriazolyl)βthymidine residue at a predetermined position were prepared according to a wellβestablished phosphite triester methodology using 2βcyanoethyl phosphoramidites of a 4β(1,2,4βtriazolyl)βsubstituted thym
The synthesis of oligodeoxynucleotides containing 4-thiothymidine residues
β Scribed by Theo T. Nikiforov; Bernard A. Cornolly
- Publisher
- Elsevier Science
- Year
- 1991
- Tongue
- French
- Weight
- 282 KB
- Volume
- 32
- Category
- Article
- ISSN
- 0040-4039
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β¦ Synopsis
Olgodeoqnucleotides contarnug 4-thothymzdm residues were obtauwdusing the ph~?sphoramtdttes of 2 and J Treatment with CHJOSK tn EtOH was used to rntroduce the I-throketo $mctron at the olqodeoqnucleotrde level.
Recently, we have reported the synthesis of 5'-0-(4,4'-dimethoxytrity1)-4-throcyanatothymidine
π SIMILAR VOLUMES
Summarv-A 5'-O-monomethoxytritylthymidine-3'-S-thiophosphoramidite (3) has been used to prepare oligodeoxynucleotides containing 3'-thiothymidine on a solid phase support. The intermediate thiophosphites are most efficiently oxidised using tetrabutylammonium periodate. Polynucleotide analogues conta