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The substrate profiles of the acidic and slightly basic horseradish peroxidases

✍ Scribed by S. Marklund; P.-I. Ohlsson; A. Opara; K.-G. Paul

Book ID
115733739
Publisher
Elsevier Science
Year
1974
Weight
516 KB
Volume
350
Category
Article
ISSN
0005-2744

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A simple and rapid technique for simultaneous separation of the acidic and basic isoenzymes of horseradish peroxidase is described. Upon application of a potential gradient on the electrolyte system composed of Pharmalyte (pH 2.5-5.0) histidine (p17.64), and Ampholyte (pH 9-(1), the acidic and basic