✦ LIBER ✦
Single-step, rapid separation of acidic and basic isoenzymes from commercial horseradish peroxidase
✍ Scribed by Aurel Popa Wagner; Eugenia Psarrou; Lieselotte Popa Wagner
- Publisher
- Elsevier Science
- Year
- 1983
- Tongue
- English
- Weight
- 194 KB
- Volume
- 129
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
A simple and rapid technique for simultaneous separation of the acidic and basic isoenzymes of horseradish peroxidase is described. Upon application of a potential gradient on the electrolyte system composed of Pharmalyte (pH 2.5-5.0) histidine (p17.64), and Ampholyte (pH 9-(1), the acidic and basic isoenzymes with pI 4.0-8.4 of horseradish peroxidase accumulated at the two interphases generated by this arrangement, with a recovery of 75 ± 5%. Some advantages, such as rapidity, simplicity, low cost, good yields, and others, of this system over existing ones are outlined.