N,NΠ-Dicyanomethyl-o-phenylenediamine was synthesized with a 90% yield by a reaction of o-phenylenediamine with chloroacetonitrile in triethylamine. Our experimental results showed that it was the effective fluorogenic substrate for horseradish peroxidase (HRP) and hemin. The K m for the HRP system
β¦ LIBER β¦
New fluorogenic substrates for horseradish peroxidase: Rapid and sensitive assays for hydrogen peroxide and the peroxidase
β Scribed by Kiyoshi Zaitsu; Yosuke Ohkura
- Publisher
- Elsevier Science
- Year
- 1980
- Tongue
- English
- Weight
- 417 KB
- Volume
- 109
- Category
- Article
- ISSN
- 0003-2697
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