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Sensitive assays for trypsin, elastase, and chymotrypsin using new fluorogenic substrates

✍ Scribed by M. Zimmerman; B. Ashe; E.C. Yurewicz; G. Patel

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
297 KB
Volume
78
Category
Article
ISSN
0003-2697

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✦ Synopsis

Sensitive fluorogenic substrates for trypsin, chymotrypsin, and elastase were prepared. These substrates are amides of an acyl amino acid or peptide with 7-amino-4-methyicoumarin (AMC). The substrates with their respective K,,,/K, ratios given in parentheses are: for chymotrypsin, glutaryl-Phe-AMC (78) and Ala-Ala-Phe-AMC TFA (1660); for trypsin, benzoyl-DL-Arg-AMC (800) and Carbobenzoxy (Cbz)-L-Arg-AMC (5300); for elastase, N-acetyl-Ala-Ala-Pro-Ala-AMC (15,000). The detection limits obtained by using the best substrates and short incubation times are: chymotrypsin, 25 ng; trypsin, 5 ng; and elastase, 2 ng.

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