Fluorogenic Substrates Containing 7-Amino-4-methyl-2-quinolinone for Aminopeptidase M, Chymotrypsin, Elastase and Trypsin, Determination of Enzyme Activity
✍ Scribed by Tzougraki, Chryssa ;Noula, Catherine ;Geiger, Reinhard ;Kokotos, George
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 432 KB
- Volume
- 1994
- Category
- Article
- ISSN
- 0947-3440
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✦ Synopsis
Abstract
Several new fluorogenic substrates for four proteases were synthesized by coupling the highly fluorescent 7‐amino‐4‐methyl‐2‐quinolinone (AMeq) with appropriate amino acids and peptides. Compounds HCl · H‐Ala‐NH‐Meq (1) and HCl · H‐Leu‐NH‐Meq (2) are substrates for aminopeptidase M, Glt‐Tyr‐NH‐Meq (3) and Glt‐Leu‐Tyr‐NH‐Meq (4) for chymotrypsin, Boc‐(Ala)~4~‐NH‐Meq (5) for elastase and Z‐Arg‐NH‐Meq (6) for trypsin. The kinetic parameters for the hydrolysis of the new substrates were determined and showed that each new substrate is suitable for a convenient fluorometric assay of the corresponding enzyme. magnified image