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The Subcellular Distribution and State of the Elongation Factor Tu in Extracts of Escherichia coli B

✍ Scribed by ANTHONY V. FURANO


Book ID
115115356
Publisher
John Wiley and Sons
Year
1976
Tongue
English
Weight
817 KB
Volume
64
Category
Article
ISSN
1432-1327

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Bulk preparation and crystallization of
✍ Marilyn Yoder; Clare Tores; Robin Corelli; Frances Jurnak πŸ“‚ Article πŸ“… 1985 πŸ› Elsevier Science 🌐 English βš– 426 KB

A simple procedure for the preparation of lo-500 mg of the Escherichia coli elongation Tu-Ts complex is described. The protocol is based on the separate purification and quantitation of EF-Tu-GDP and EF-Ts, followed by mixing of equimolar amounts of each protein and removal of the displaced GDP by d