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The structure of the biological repeating unit of the O-antigen of Hafnia alvei O39

✍ Scribed by Ewa Katzenellenbogen; Elzbieta Romanowska; Nina A. Kocharova; Alexander S. Shashkov; Grigory M. Lipkind; Yuriy A. Knirel; Nikolay K. Kochetkov

Publisher
Elsevier Science
Year
1990
Tongue
English
Weight
567 KB
Volume
203
Category
Article
ISSN
0008-6215

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✦ Synopsis

On mild acid-catalysed degradation of the lipopolysaccharide from Hafnia alvei O39 followed by gel filtration of Sephadex G-50, the O-specific polysaccharide and three oligosaccharides were obtained, which represent the core substituted with 0-2 O-antigen repeating-units. On the basis of sugar and methylation analyses, 13C-n.m.r. data, solvolysis of the polysaccharide with anhydrous hydrogen fluoride, and computer-assisted 13C-n.m.r. analysis of the Smith-degraded polysaccharide, it was concluded that the biological repeating unit of the O39 antigen was Formula; see text

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