The O-specific polysaccharide of Hafnia alvei strain 1216 is composed of o-galactose, o-glucuronic acid, 2-acetamido-2-deoxy-o-glucose, 3,6-dideoxy-3-[CR)-3-hydroxybutyramidolo-glucose, and O-acetyl groups in the ratios 1: 1: 2: 1: 1. On the basis of sugar and methylation analyses of the intact and
Structure of the Hafnia alvei strain PCM 1188 O-specific polysaccharide
✍ Scribed by Andrzej Gamian; Ewa Katzenellenbogen; Elzbieta Romanowska; JoséManuel García Fernández; Christian Pedersen; Jacques Ulrich; Jacques Defaye
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 557 KB
- Volume
- 277
- Category
- Article
- ISSN
- 0008-6215
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✦ Synopsis
The lipopolysaccharide was extracted from cells of Hafnia alvei PCM 1188 strain and, after mild acid hydrolysis, the O-specific polysaccharide isolated and characterized. On the basis of sugar and methylation analysis, FAB mass spectrometry and NMR spectroscopy of the polysaccharide and oligosaccharides obtained after Smith degradation, or solvolysis with anhydrous hydrogen fluoride, the repeating unit of the O-specific polysaccharide was shown to be the pentasaccharide: [formula: see text]
📜 SIMILAR VOLUMES
The O-specific polysaccharide of the lipopolysaccharide from H. alvei strain 38 has been established by NMR spectroscopy (13C and 1H) and methylation analysis to have the repeating unit-->4)-beta-D-ManpNAc-(1-->4)-alpha-D-GlcpNAc(1-->.
The O-specific polysaccharide of the lipopolysaccharide produced by Hafnia alvei strain 1220 contained D-glucose, D-galactose, N-acetyl-D-glucosamine, N-acetyl-L-fucosamine (2-acetamido-2,6-dideoxy-L-galactose), glycerol, and phosphate. It was proved by composition and methylation analyses, Smith de
The O-specific polysaccharide of Hafnia alvei 1205 contained D-glucose, D-galactose, 2-acetamido-2-deoxy-D-glucose, 4-acetamido-4,6-dideoxy-D-glucose (Qui4NAc), glycerol, phosphate, and O-acetyl groups. On the basis of 1D and 2D shift-correlated homonuclear and 13C-1H heteronuclear NMR spectroscopy,
The O-specific polysaccharide of Hafnia alvei strain 1204 has a hexasaccharide repeating unit containing D-mannose, D-glucuronic acid, 2-acetamido-2-deoxy-o-glucose, 2-acetamido-2-deoxy-D-galactose, and 3,6-dideoxy-3-formamido-o-glucose (Qui3NFo) in the ratios 2:1:1:1:1 as well as O-acetyl groups. O