Anthers of the Solanum tuberosum genotype H3703 were cultured on medium containing equimolar concentrations of sucrose or maltose. It was found that significantly more pollen embryos became plants after culture on maltose and hence the yield of plants per 100 anthers cultured increased significantly
The statistical analysis of potato anther culture data
โ Scribed by W. Powell; M. Coleman; J. McNicol
- Publisher
- Springer
- Year
- 1990
- Tongue
- English
- Weight
- 391 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0167-6857
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โฆ Synopsis
Methods for the production and utilisation of haploids have been developed for a range of crop plants. Genetical and environmental factors are known to influence both the rate of haploid induction and the mode of regeneration. Investigations designed to examine the parameters that influence haploid production from anthers are usually based on the overall percentage of responding anthers. Previous studies have rarely taken into account the frequency or distribution of anther culture response within a potato flower. In this paper the binomial, poisson and multiplicative binomial distributions are used for the first time to describe the distribution of anther culture response within a Solanum tuberosum flower.
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Three Andean tetraploid potato genotypes (2n= 48) and 7 anther-derived dihaploids (2n=24) originating from two of the tetraploids were used in anther culture. Relative number of embryos/vial was significantly higher when the anther culture media was gelatinized with 3% potato starch than when Gelrit
Anthers of diploid genotypes of Solanum tuberosum capable of androgenesis were cultured on different media to examine the effect on induction of pollen embryogenesis of 2,4-D and lactose. Anthers cultured in callogenic medium with 2,4-D and sucrose produced pollen derived embryoids only exceptionall
The role of ethylene in embryogenesis of cultured potato anthers was studied indirectly by testing various substances known to affect ethylene formation. The reducing agents ascorbic acid and L-cysteine prevented browning of anther cultures and significantly stimulated embryogenesis. Embryogenesis w